Fig. 4

Pulse-chase experiment on YPXL mutant and WT viruses showing the virus production is affected after TSG101 or EAP20 is knocked down. a Total cellular lysates and supernatants were harvested at time points 0, 1.5 and 3 h post pulsing under siTSG101, siEAP20 or siControl conditions followed by IP and SDS-PAGE using p24 specific antibody. The predicted viral protein sizes are noted at the left. b The release ratio was defined as the viral protein in the virions divided by that in the total cell lysates. The ratio at each time point was then normalised against that of YPXL mutant under siControl conditions at 3 h. The error bars represent the SEM and were calculated based on three replicates.