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Fig. 2 | Retrovirology

Fig. 2

From: Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production

Fig. 2

Reduction in infectious virus production by EAP45 mutants. a Domain organisation of EAP45 and mutants used in this study. b Western blot detecting the expression of GLUE as a 16 kDa protein by a polyclonal rabbit anti-EAP45 antibody. c HA-tagged wt or H0M EAP45 expression plasmids were transfected into the cells. The level of expression was visualised by Western blot using anti-HA antibody. Tubulin is served as a loading control. d Cell viability assay performed as described for Fig. 1a. e Productions of infectious wild type viruses upon co-transfection of EAP45 mutants harvested 96 h post-transfection are shown. Transfections and analyses were performed as in Fig. 1. Error bars representing the SEM from up to six replicates are shown.

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Retrovirology

ISSN: 1742-4690

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