Table 2 Sequences of shRNA used to deplete the corresponding targets, and primers used for real time PCR

pLKO-1 shRNA IRF-1 target sequences

 IRF-1

CGTGTGGATCTTGCCACATTT

 IRF-1

CCTCTGTCTATGGAGACTTTA

 IRF-1

GCAGATTAATTCCAACCAAAT

 IRF-1

AGATGCTAAGAGCAAGGCCAA

 IRF-1

GCGTGTCTTCACAGATCTGAA

TetOn-pLKO-1 shRNA

 hiNOS

Forward: 5′-CCGGAACATTGCTGTGCTCCATAGTCTCGAGACTATGGAGCACAGCAATGTTTTTTG-3'  

 hiNOS

Reverse: 5′-AATTCAAAAAAACATTGCTGTGCTCCATAGTCTCGAGACTATGGAGCACAGCAATGTT-3'

 GFP

Forward: 5′-CCGGCAGCCACAACGTCTATATCATCTCGAGATGATATAGACGTTGTGGCTGTTTTTG-3'

 GFP

Reverse: 5′-AATTCAAAAACAGCCACAACGTCTATATCATCTCGAGATGATATAGACGTTGTGGCTG-3'

Real time PCR primers

 hGAPDH

Forward: 5′-GTGAAGGTCGGAGTCAACGG-3′

Reverse: 5′-AGTGATGGCATGGACTGTGG-3′

 hiNOS

Forward: 5′-ACAACAAATTCAGGTACGCTGTG-3′

Reverse: 5′-TCTGATCAATGTCATGAGCAAAGG-3′

 IκBα

Forward: 5′-TGTCTACACTTAGCCTCTATC-3′

Reverse: 5′-TCTGTGAACTCCGTGAACTC-3′

 IRF-1

Forward: 5′-GAACTCCCTGCCAGATATCGAG-3′

Reverse: 5′-TGCTCTTAGCATCTCGGCTGG-3′

 SOCS1

Forward: 5′-TTTTTCGCCCTTAGCGTGA-3′

Reverse: 5′-AGCAGCTCGAAGAGGCAGTC-3′

 Tax

Forward: 5′-CGGATACCCAGTCTACGTGT-3′

Reverse: 5′-GAGCCGATAACGCGTCCATCGATG-3′