Fig. 7

Analysis of ROD14 Env cytoplasmic tail revertants. a Schematic representation of the Env proteins from HIV-1 (isolate BH10), ROD10, and the chimeric protein E1C10, containing the extracellular and transmembrane domains of BH10 and the cytoplasmic domain of ROD10 Env. Similar chimeras were produced containing the BH10 extracellular domain and the cytoplasmic (C) domain of RO10, RODD14, Rev A and Rev B. MSD is membrane-spanning domain. The ability of each Env to counteract tetherin restriction is also noted [35]. b HIV-1 VLPs were produced in 293A cells in the presence of tetherin (100 ng), alone or together with each Env (2 μg). The percent VLP release was determined as described previously with results normalized to the no tetherin control, for n = 4 independent experiments, p < 0.05 (*).