Skip to main content
Fig. 6 | Retrovirology

Fig. 6

From: Determinants in HIV-2 Env and tetherin required for functional interaction

Fig. 6

ROD14 Env reacquires anti-tetherin activity when passaged in culture. a Schematic of sequences in 9 independent clones isolated from ROD10(14 Env) infected JLTRG cells, with mutations shown relative to where they occur in either the gp120 or gp41 subunits. The mutations designated Rev A were found in all 9 clones, Rev B was in 7 out of 9 clones, and Rev C1 and C2 both occurred in 2 out of 9 clones each. All 9 clones retained the original ROD14 Env mutations R422 and T598, associated with loss of anti-tetherin activity. MSD is membrane-spanning domain. b The indicated Env clones were tested at two separate amounts, 2 and 4 μg (×2), in a VLP release assay in 293A cells in the presence of tetherin, as previously described. Results were normalized to the no tetherin control, for n = 3 independent experiments, p < 0.05 (*). c Revertant Envs were GFP-tagged and tested for their ability to co-IP with tetherin in 293T cells, as previously described. d Indicated Env clones, including single and double substitutions in the ROD14 Env backbone, were tested in a VLP release assay in 293A cells in the presence of tetherin. Results were normalized to the no tetherin control, for n = 3 independent experiments, p < 0.05 (*).

Back to article page