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Fig. 1 | Retrovirology

Fig. 1

From: Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms

Fig. 1

Detection of KoRV infection in DERSE cells. DERSE cells were infected with KoRV released from 293T cells transiently transfected with pKoRV522, and they showed GFP expression as indicated by fluorescence microscopy (middle of the figure), indicative of KoRV replication. Supernatants from the infected DERSE cells (after 5 passages) were used to secondarily infect fresh 293T cells (bottom of the figure). The infected 293T cells were green and SDS-PAGE and western blot for KoRV Gag protein (bottom right) showed that the cultures were expressing KoRV Gag protein, indicative of productive infection. Uninfected 293T and DERSE cells (see Fig. 2) showed no background green fluorescence.

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