Fig. 4

Primary Vpu isolates maintain the ability to decrease surface expression of CD4 and BST-2 in a CRL-dependent and independent manner. a Primary CD4⁺ T cells were either mock infected or infected at an MOI = 1 with DHIV-GFP (Nef−/Vpu−), DHIV-GFP (Nef−/Vpu+) or viruses encoding Vpu taken from either a transmitted/founder (T/F) (DHIV-GFP WITO (Nef−/Vpu+)) or a chronic carrier (CC) primary isolate (DHIV-GFP WARO (Nef−/Vpu+)). The cells were treated with either DMSO or 500 nM MLN4924 48 h post infection. 24 h post MLN treatment, the cellular GFP negative and positive populations (red and blue histograms) were assessed for surface levels of CD4 and BST-2 via flow cytometry. An IgG control was included to set the baseline for positive staining (gray shaded histogram). b Relative CD4 and BST-2 surface levels were quantified, normalized, and scored statistically as described in Fig. 2b.