Fig. 3
NK cell dependent target cell killing. DEREG mice were infected with FV and mice were either Treg-depleted by repeated injections of DT or NK cell-depleted or Treg- and NK cell-depleted. At 10 dpi mice received 5 × 105 CFSE-labeled RMA/S. Two days later (12 dpi) cells were obtained by peritoneal lavage and analyzed by flow cytometry (a). At least three independent experiments were performed with at least five mice. Individual percentages and mean (±SEM) values were indicated by dots and bars. b DEREG mice were infected with FV and several mice were Treg-depleted by repeated injections of DT. At 10 dpi splenic NK cells were isolated and adoptively transferred into FV-infected mice. Four days post transfer mice were sacrificed and viral loads were analyzed in the spleen. FV-infected mice, which did not receive any cell transfer, were used as control. At least four mice per group were tested. Statistically significant differences between the different transferred NK cells were analyzed via Mann–Whitney test and are indicated by single asterisk for p < 0.05 and double asterisk for p < 0.01.