Figure 6
From: Reactivation of latent HIV-1 provirus via targeting protein phosphatase-1
Effect of SMAPP1 on cellular protein expression. a Venn diagram of proteins from CEM T cell proteins treated with 10 μM SMAPP1 or DMSO as a control. Cellular proteins were extracted, trypsinized and tryptic peptides were separated by ion exchange chromatography and then analyzed by high resolution mass spectrometry. Proteins were identified by Proteome Discoverer 2.1. Proteins eluted in 0, 25, 50, 100, 250 and 500 NaCl were combined. b Venn diagram of protein phosphatase-related proteins identified in CEM T cells. c, d Peptides detected in 100 mM salt fraction were quantified using SIEVE 2.1 and exported to Ingenuity software for the analysis of protein networks. Transcription (c) and protein phosphatase (d) networks are shown. Up-regulated genes are colored in red and down-regulated genes are colored in green.