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Figure 5 | Retrovirology

Figure 5

From: HIV inhibits endothelial reverse cholesterol transport through impacting subcellular Caveolin-1 trafficking

Figure 5

Influence of Nef on HDL mediated cholesterol efflux from HAECs. a Expression levels of Nef from HIV infected cells. Cell lysates and TCA and acetone precipitated culture media supernatants from infected cells were subjected to immunoblotting to detect Nef. Lanes 1 through 3 represent released Nef from HIV infected cells and Lanes 4 through 6 from HIV infected cell lysates. b HAECs were treated with recombinant Nef at indicated concentrations for 72 h, and then HDL mediated cholesterol efflux was analyzed. *P < 0.05 compare with control cells, Mock non-treated HAECs. c HAECs were exposed to recombinant Nef (0–150 ng/ml) for 48 h. The expressions of ABCA1, ABCG1, SR-BI and Cav-1 as well as eNOS from above HAECs were examined by Western blotting. Representative Western blots are shown.

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