Figure 2
Sodium carbonate extraction and sucrose gradient isolation of caveolae membrane fractions. HAECs were homogenized and separated by a detergent-free (Na2CO3) based discontinuous sucrose density centrifugation. a Twelve fractions were collected from the gradient going from low to high density and were analyzed by Western blotting with antibody for specific markers of caveolae (Cav-1, Flotilin-1) β-tubulin (cytoplasmic components). b Density of each fraction was measured.