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Figure 1 | Retrovirology

Figure 1

From: HIV inhibits endothelial reverse cholesterol transport through impacting subcellular Caveolin-1 trafficking

Figure 1

HIV impairs HDL mediated cholesterol efflux from HAECs. a HAECs co-cultured with HIV infected ACH2, U1HIV and HUT-78HIV, or b uninfected parental cell lines A3.01, U937 or HUT-78. HDL induced cholesterol efflux from HAEC was examined. c HAECs and HUT-78HIV were co-cultured with different HAEC/HUT-78HIV ratios 1:1 or 1:5 for 7 days, and cholesterol efflux measured. d HAECs were co-cultured with HUT-78HIV at ratio 1:3 and HDL mediated cholesterol efflux at days 3 and 7 were analyzed. e U1HIV and control cells U937 were pretreated with PMA (10−8 M) for 48 h before co-culture, and HAECs were co-cultured with those cells for 7 days and further subjected to cholesterol efflux examination. f The expression levels of ABCA1, ABCG1, SR-B1, and Cav-1 in HAECs. HAECs were co-cultured with HIV infected cells ACH2, HUT78HIV and U1HIV for 5 days. The expressions of ABCA1, ABCG1, SR-BI and Cav-1 HAECs were examined by Western blotting. Representative Western blots are shown. Mock HAECs cultured alone, w/sti PMA treated; *P < 0.05 compared with control cells.

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