Figure 6

Co-localization of endogenous HBZ with intracellular factors in C5MJ cells. C5MJ cells were reacted in a pairwise combination with the 4D4-F3 anti HBZ mAb antibody, and either polyclonal rabbit anti-CBP, anti-JunD, anti-p300, anti-CREB2, or anti-nucleolin antibodies. Anti-HBZ mAb was revealed by Alexa fluor 546-labeled goat anti-mouse IgG (red), whereas the other rabbit antibodies were revealed by Alexa fluor 488-labeled goat anti-rabbit antibodies (green). The MERGE column panels represent the merge between the Alexa fluor 546 and the Alexa fluor 488 signals. A co-localization of HBZ with either one of the intracellular factors results in a yellow color. The reference of intensity (ROI) drawn along mid-nucleus level of the merge image (insert) is represented by red and green peaks in the histogram (red for HBZ and green for the intracellular factor).