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Figure 3 | Retrovirology

Figure 3

From: Localization, quantification and interaction with host factors of endogenous HTLV-1 HBZ protein in infected cells and ATL

Figure 3

Subcellular localization of endogenous HBZ in HTLV-1 chronically infected and in ATL tumor cells. C5MJ and ATL-2s cell lines and PH961 patient PMBC were stained with the 4D4-F3 antibody, followed by Alexa fluor 546-labeled goat anti-mouse antibody and analyzed by confocal microscopy. 293T cells transiently transfected with myc-HBZ cDNA plasmid and the human T cell line Jurkat were used as positive and negative controls, respectively. Right panels show a representative single cell of each corresponding field. The percentage of HBZ-positive cells was calculated by counting at least 200 cells. Results were ATL-2s, 100%; C5MJ, 75%; PH961, 80%; 293T.HBZ, 55%.

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