Figure 8

Tax1-responsive elements in the CD83 promoter. a Luciferase reporter plasmids carrying the wild-type (−537 to +41) and its 5′ deletion mutants (−285 to +41, −146 to +41, −101 to +41, and −29 to +41) of the CD83 promoter were transfected into Jurkat cells along with the Tax1 expression plasmid (pMT-2Tax). Cells were cultured for 48 h and analyzed for luciferase activity. The results were normalized based on protein content. b Nucleotide sequences of the CD83 promoter (−101 to −1 with respect to the transcription start site). Possible NF-κB binding sites (NF-κB1 and NF-κB2) are underlined. c Schematic representation of the possible NF-κB binding sites in the CD83 promoter is shown with lowercase letters indicating mutated sequences. d Reporter plasmids carrying the wild-type CD83 promoter or mutant promoter were transfected into Jurkat cells along with the Tax1 expression plasmid (pMT-2Tax). Cells were cultured for 48 h and analyzed for luciferase assay. The results shown are as the mean ± SE after normalization based on protein content.