Figure 4

Quantitation of cell-associated CD83 and sCD83. The HTLV-I⁺ T cell lines (MT-2 and HUT-102) and T cell lines from either ATL patients (ATL-026, ATL-056, ATL-084, ILT-H2) or a HAM/TSP patient (OKH4) were cultured in a volume of 1 ml/well for 3 days in vitro. Cells and culture supernatants were separated by centrifugation, and the cells were lysed with 1 ml of a lysing buffer. The levels of CD83 in the culture supernatants or in the cell lysates were determined by ELISA. Results are shown as the mean ± SD of CD83 of triplicate cultures.