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Figure 6 | Retrovirology

Figure 6

From: Identification of novel monocistronic HTLV-1 mRNAs encoding functional Rex isoforms

Figure 6

Functional analysis of Rex isoforms using the pcGagRXRE reporter plasmid. a Schematic representation of the pcGagRXRE reporter plasmid [17]. b Expression of HIV-1-p55Gag and p24Gag after the co-transfection of HLTat cells with 0.5 µg of pcGagRXRE and 0.5 µg of BlueScript (negative control) or increasing amounts (0.1, 0.2 and 0.5 µg) of pMH-Rex, pMH-Rexa, pMH-Rexb and pMH-Rexc. Immunoblots were prepared and probed with mouse anti-HIV-1 p24 monoclonal antibody (1:500, Chemicon) and mouse anti-α-tubulin monoclonal antibody (1:2,000; Sigma-Aldrich) as described in the legend to Figure 5.

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