Figure 4

Viruses with P/SDI/V α4β7 binding motifs are more reactive with the integrin. a T/F viruses with P/SDI/V showed a higher dependence on the integrin compared to those with LDI/L motifs (n = 4, blue) (*p = 0.029; Mann–Whitney test). b Mutation of CAP8 T/F from LDI to PDI and CAP256 T/F from LDL to SDI resulted in a significant increase in α4β7 dependence (*p = 0.02 for both, paired t test). Bars represent three independent experiments and error bars indicate the SEM. c CAP8 and CAP256 mutants also showed increased α4β7 binding compared to the wild-type in the direct assay using p24-FITC MFI as a read-out, representative of three independent experiments.