Figure 3

Bacterial vaginosis and genital cytokines associated with α4β7-dependent T/F viruses. a T/F viruses of CAP225, CAP88, CAP200 and CAP206 showed high (>50%, indicated by the dotted line) α4β7 dependence (green) while the remaining four showed lower α4β7 dependence (blue). Dependence on α4β7 was determined using Act-1 mAb inhibition. Bars represent the mean of four independent experiments, with error bars indicating SEM. b Individuals infected with T/F viruses that had higher dependence on α4β7 were significantly more likely to be BV positive at the time of infection (p = 0.029; Mann–Whitney test). c Concentrations of cytokines in CVL (n = 31) and plasma (n = 30) were determined and correlated with T/F virus dependence on α4β7 for replication. IL-7, IL-8 and IL-1α showed significant univariate correlations in the CVL. In contrast only eotaxin was significantly associated in plasma (shown in the grey boxes) while IL-8, IL-1α and IL-7 showed no correlation in plasma. No significance was maintained at a multivariate level. Relevant p values and Spearman’s coefficients are shown where *p < 0.05.