Figure 2

HIV dependence on α4β7 for replication over the course of infection. a Kinetic growth curves of IECs from CAP88 T/F, 1 month, 12 and 39 months post-infection (green lines) in the presence of α4β7 inhibitory mAbs Act-1 (blue) and HP2/1 (red) or anti-CD4 mAb (purple) measured as p24 (ng/ml) over 10 days. Inhibition by Act-1 and HP2/1 was partial at the point of exponential growth of the virus control and did not differ between the two mAbs. Curves are representative of three independent experiments, each one in triplicate and error bars representative of SEM. b Longitudinal IECs (from 3 individuals) including the T/F virus and multiple 2–39 months post-infection viruses were tested for their dependence on α4β7 for viral replication. The percentage inhibition by HP2/1 (red) and Act-1 (blue) is expressed as the difference in p24 concentration between HP2/1 or Act-1 treated and untreated cells at the point of exponential viral growth. Differences in dependence across all time points in each of these three individuals were significant by a repeated measures ANOVA (p < 0.0001) as well as between CAP88 T/F and 1 month p.i, (**p < 0.001) CAP200 T/F and 6 months p.i. (*p < 0.01) and CAP206 T/F and 2 months p.i (**p < 0.001). Pairwise comparisons were adjusted by the Tukey method. Bars represent means of between two and three independent experiments with the error bars indicating SEM.