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Figure 3 | Retrovirology

Figure 3

From: Nuclear matrix protein Matrin 3 is a regulator of ZAP-mediated retroviral restriction

Figure 3

Effects of other major nuclear matrix proteins on zap activity. Distinct nuclear matrix proteins regulate ZAP effects on a HIV-1 or HR’-CMV-luc and b MoMuLV. 293TrexhZAP2 cells were transfected with control siRNA (Ctrl) or siRNA against Matrin 3 (Matrin 3 KD), HNRNPU (HNRNPU KD), SFPQ (SFPQ KD), or Lamin A/C (Lamin A/C KD) for two consecutive days. Subsequently on the following day, cells were infected with HIV-luc or HR’-CMV-luc then treated with (ZAP) or without doxycycline treatment at 200 ng/ml. Cells were then harvested 2 days postinfection and lysates were subjected to luciferase assays to measure Nef-luc reporter activity. Fold restriction was calculated from dividing Ctrl siRNA values by the results of each treatment indicated. Data presented are the mean RLU/mg values of three independent experiments done in triplicate ±SD (HIV and MoMuLV) and two experiments done in triplicate ±SD for HR’-CMV-luc. Statistical analyses were done to test for differences between ZAP and ZAP induced in cells treated with siRNA. P values ≤0.05 are shown.

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