Figure 7

HDAC6 interacts with Vif and A3G in cells. a Cell lysates from CEM.NKR.CCR5 cells expressing endogenous A3G and HDAC6 together with overexpressed Vif were subjected to co-IP with anti-HDAC6 Ab, followed by immunoblotting with HDAC6, A3G or Vif Abs. b Cell lysates from HEK 293T cells expressing non-tagged A3G alone or co-expressed with HA-Vif, EGFP-wt-HDAC6 or EGFP-HDAC6-ΔBUZ, were subjected to co-IP with anti-HA Ab from cell lysates treated or not with RNAse A (right and left, respectively), followed by immunoblotting with HDAC6, A3G or Vif Abs. In a, b, actin or α-tubulin is the control for total protein, and when detected endogenous HDAC6 and IgG Heavy- or Light-chains are indicated. Data shown are representative of three independent experiments.