Figure 5

HDAC6 and A3G co-localize along microtubules in cells. a A series of confocal images, xy sections, shows expression patterns for endogenous HDAC6 and α-tubulin, and overexpressed A3G-3xHA. Different merged images are shown to display endogenous HDAC6/overexpressed A3G-3xHA co-distribution and co-distribution of this complex in the cytoplasm or upon MTs (α-tubulin). In the zoom area (black-bordered inset) is shown the expression of these proteins and their co-distribution, as indicated. b Series of confocal images, xy sections, shows expression pattern for overexpressed EGFP-wt-HDAC6 or A3G-3xHA and α-tubulin. Merged image is shown to display EGFP-wt-HDAC6/A3G-3xHA co-distribution in cytoplasm or upon MTs (α-tubulin). In the zoom area (black-bordered inset) is shown the expression of these proteins and their co-distribution, as indicated. c Series of confocal images, xy sections, shows expression pattern for overexpressed HA-wt-HDAC6, HA-HDAC6-ΔBUZ or A3G-Flag and α-tubulin. Merged image shows HA-wt-HDAC6/A3G-Flag (top) or HA-HDAC6-ΔBUZ/A3G-Flag (bottom) co-distribution in cytoplasm or upon MTs (α-tubulin). From a to c, data are representative of three independent experiments in HEK 293T cells.