Figure 3

SAMHD1-mediated RNA degradation inhibits viral cDNA synthesis. Differentiated U937 cells-expressing SAMHD1 variants were infected with FIV-GFP (MOI = 1) (a), F-MLV-GFP (MOI = 5) (b), or EIAV-GFP (MOI = 1) (c) for 2 h. At 24 h post-infection, viral cDNA synthesis was monitored by qPCR using primers specific for gfp (a, c) or MLV late RT products (b). Data were normalized against an internal mdm2 control. Data are expressed as the mean ± SD from three independent experiments in triplicate. *p < 0.05 compared with the mock-transfected control (two-tailed Student’s t test).