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Fig. 5 | Retrovirology

Fig. 5

From: Functional bottlenecks for generation of HIV-1 intersubtype Env recombinants

Fig. 5

Cloning of only gp120 sequences of intersubtype envA/D recombinants into NL4-3 backbone and the resultant envelope function assay. Intersubtype A/D recombinant gp120 genes were cloned, leaving gp41 of NL4-3 intact. (a) The plasmids containing recombinant gp120 sequences were tested by Veritrop assay for functionality and (b) for replication-competent virus production. Pseudoviruses produced from the plasmids were tested by ELISA (c) and Western blot (d) for the production of envelope expression

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