Fig. 2
From: Functional bottlenecks for generation of HIV-1 intersubtype Env recombinants
Systematic strategies for cloning envA/D recombinant sequences. (a) Full envA/D recombinants were cloned into a plasmid containing a near full length HIV-1 NL4-3 backbone; (b) plasmids cloned in panel A were modified by insertion of tat1, rev1 and vpu from isolate D109 to match the corresponding tat2 and rev2 sequences; (c) selected gp120 recombinants from panel B were cloned such that NL4-3 gp41 was unaltered; (d) only V1-V5 of recombinant gp120 sequences was cloned in order to preserve the consistency between C1, C5, and gp41 domains. Subtype A sequences (A115 and A91) are denoted by red lines, and subtype D (D109) sequences are denoted by green lines