Fig. 8
From: Apoptosis-induced activation of HIV-1 in latently infected cell lines
Infectious virion production after induction of replication by apoptosis. The infectivity of virions produced via apoptosis-mediated HIV-1 activation was determined using luciferase assay by exposing 1G5 cells to supernatants from cells treated with cytotoxic agents. Equal amounts (200 ng) of virus-containing supernatants, as determined by p24 assays were added to the 1G5 cells (with or without AZT, 5 μM) and luciferase activity determined. Infectious virus was produced following induction of replication with both conventional induction and apoptosis induction. For mock infections, 1G5 cells were incubated with culture supernatant of Jurkat cells treated with TNF-α. Values represented as mean ± SD (n = 3)