Fig. 6
From: Apoptosis-induced activation of HIV-1 in latently infected cell lines
Apoptosis-mediated HIV activation in G1-arrested cells. (a) Synchronization of U1 culture using simvastatin to enrich the cells in G1-phase. (b) Apoptosis in synchronized cells was induced by doxorubicin (2.5 μM), etoposide (5 μM) or vincristine (5 μM). Cells induced with PMA were used as positive control for HIV-1 activation. Apoptosis (white bars) was assessed by Annexin-V staining and HIV activation was measured by qPCR for unspliced HIV-1 RNA