Heat map of expression after stimulation of known HIV-1 restriction factors and gene candidates through the screening approach. On the left, CD4+ T cells from healthy donors were left unstimulated or stimulated with IFNα, IFNγ or αCD3/CD28. Expression levels of known restriction factors (blue), and factors identified in the screen (black) were clustered using complete hierarchical clustering. For comparison, the right panel depicts the correlation between gene expression in CD4+ T cells of HIV infected donors with setpoint viral load (R(VL)). Correlation values were taken from . Positive R(VL) values (yellow) indicate an increased gene expression correlates with an increase in patient viral load (log10 RNA copies/ml).