Titration of the effects of the gene candidates on infectious HIV-1 production. Virus release from 293T cells following transfection with 5 μg of wt or Δ3 proviral NL4-3 constructs and varying amounts of plasmids expressing the indicated candidate genes. The total quantity of transfected DNA was adjusted to 7.5 μg with empty vector. Infectious virus yield was determined by infection of TZM-bl indicator cells and is shown as percentage of that detected in the absence of candidate gene expression. Values represent averages (±SD) derived from triplicate infections and the results were confirmed in an independent experiment.