Alterations in the frequency of phenotypically naive CD4+ T-cell subsets in HIV-infected subjects. PBMCs from HIV- controls and HIV-infected subjects, RI and CI on ART, were analyzed for the frequency of total CD45RA+CCR7+CD4+ T-cells (naive-like) and with a CD25highCD127−FoxP3+ (nTregs) or CD25+CD127+FoxP3− (DP) phenotype, together with the frequency and counts of total CD25+ T-cells. Cells were gated as in Figure 2. The viability dye Vivid was used to exclude dead cells. (A-D) Shown are the frequency (left panel) and counts (right panel) of total naive-like (A), nTregs (B), DP cells (C), and total CD25+ T-cells (D) in the peripheral blood of HIV- (n = 19), RI (n = 15) and CI on ART (n = 18) subjects. Each symbol represents a different subject. The counts of CD45RA+CCR7+ subsets were calculated relative to their frequency and the CD4 counts. The Kruskal-Wallis and Dunns post-test p-values are indicated on the graphs (*, p < 0.05; **, p < 0.01; ***, p < 0.001). Clinical parameters of subjects used in these studies are included in Table 1(HIV- #01-03, 05, 07, 09–18, 20–23), Table 2 (RI# 1–15), and Table 3 (CI #01, 03–18).