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Figure 2 | Retrovirology

Figure 2

From: Mice transgenic for equine cyclin T1 and ELR1 are susceptible to equine infectious anemia virus infection

Figure 2

In vitro infection of mouse embryonic fibroblasts (MEFs) and mouse blood monocyte-derived macrophages (mMDMs) from ELR1/eCT1 transgenic mice with EIAV. (A) and (C) MEFs and mMDMs were prepared from the embryos and blood of ELR1/eCT1 mice and wild-type mice. Cellular DNA was extracted and assessed for the existence of ELR1 and eCT1 using PCR. N: negative control using MEFs and mMDMs from wild-type mice; P: positive control using equine monocyte-derived macrophages (eMDMs). (B) and (D) MEFs and mMDMs prepared from ELR1/eCT1 mice and wild-type mice, as well as eMDMs, were infected with 103 TCID50 EIAVDLV34. The cells were washed three times and then cultivated for 72 h. The viral RNA was extracted from the culture supernatant and amplified by RT-PCR using EIAV-env specific primers. In addition, total DNA was prepared from the lysates of the same sets of 72 h-cultivated cells to detect the proviral genomes of EIAV using PCR with the same primers. N: negative control using MEFs and mMDMs from wild-type mice; P: positive control using eMDMs.

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