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Figure 3 | Retrovirology

Figure 3

From: HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells

Figure 3

Siglec-1 is up-regulated on monocytes of HIV-1-infected individuals, but its expression is reduced after successful antiretroviral treatment. A. Mean number of Siglec-1 Ab binding sites per cell displayed by monocytes isolated from HIV-1-negative men and from HIV-1-infected men before or after successful antiretroviral treatment (with an initial median of 297 CD4+ T-cell count and 5 log10 HIV RNA copies/ml that changed to a median of 505 CD4+ T-cell count and 1.7 log10 HIV RNA copies/ml after treatment). Graph shows mean values and SEM from nine HIV-1-negative individuals and 16 HIV-1-infected individuals. Man Whitney t-test was used to compare differences between HIV-1-negative individuals and HIV-1-infected individuals. Paired t-test was used to assess differences between HIV-1-infected men before or after successful antiretroviral treatment. B. Uptake of fluorescent VLPs by monocytes isolated from HIV-1-infected individuals before (red dots) and after (green dots) antiretroviral treatment. Cells were pulsed with VLPs for 3 h at 37°C and assessed by FACS. Graph shows mean values and SEM from 16 HIV-1-infected individuals. C. Uptake of HIV-1NL4–3 by monocytes isolated from HIV-1-infected individuals before and after antiretroviral treatment. Cells were cultured with HIV-1 for 4 h at 37°C, washed and lysed to measure p24Gag by an ELISA. Graph shows mean values and SEM of the 13 individuals from which we recovered enough monocytes to perform this assay. D. HIV-1 transmission from monocytes isolated from HIV-1-infected individuals before and after antiretroviral treatment to a reporter CD4+ cell line cultured at a ratio 5:1. Cells were pulsed with HIV-1NL4–3 as in Figure 2D. Graph shows mean values and SEM from the same individuals of panel C.

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