Effect of ABX464 on cellular and HIV-1 RNA splicing. a Heat map showing percent-spliced-in (psi) shifts for the 264 alternate splicing events in positive control fibroblasts (fibroblasts) and their iPSCs (stem) untreated, PBMCs untreated (Cells) or PBMCs treated by either DMSO without drug (DMSO) or with various compounds (ABX35, ABX36, ABX273, ABX388, ABX387 ABX402, ABX415, ABX449, ABX464 and Darunavir). b Scatter plots comparing splicing changes for the 264 exons in PBMCs treated with ABX464 versus DMSO (left panel) or stem cells differentiated into fibroblast (middle panel). The right panel shows a scatter plot comparing psi changes for the 264 exons induced by ABX464 to those induced during stem cell differentiation. Pearson correlations (R values) are shown. c Visualisation of HIV-1 splice junctions after capture and sequencing of HIV-1 RNAs extracted from PBMCs infected with the YU2 strain, either untreated (DMSO) or treated with ABX464 (ABX464) 3 days pi (d3) or 6 days pi (d6) using 454 pyrosequencing (according to GS junior method manual). Estimate of the distribution of read mappings, positions of known acceptor (A1, A2, A3, A4a, A4c and A7) and donor splice sites (D1a, D2G4, D3 and D4), the exon-exon junctions (the line of accolade which is dependent of the junction abundance) and the known coding regions of HIV-1, are shown.