ABX464 Inhibits HIV-1 production in PBMC- and macrophages-infected cells. a Drawing of 10-chloro-2,6-dimethyl-2H-pyrido[3′,4′:4,5]pyrrolo[2,3-g]isoquinoline (IDC16), 8-chloro-N-(4-(trifluoromethoxy)phenyl)quinolin-2-amine (ABX464) and 8-chloro-N-glucuronide-N-(4-(trifluoromethoxy)phenyl)quinolin-2-amine) (ABX464-N-glucuronide) compounds. b HIV-1 strain Ada-MR5 was used to infect triplicate of activated PBMCs from different donors (stimulated for two days with PHA and IL2) in the absence or presence of increasing concentrations of ABX464. Supernatant was harvested 6 days post-infection (pi) and viral capsid protein p24 antigen was quantitated using a standard ELISA protocol. Each point represents 5 donors. c Concurrently, cell viability was measured by MTS assay after 6 days of incubation and cytotoxicity was indicated as percentage as compared with untreated cells. d Histogram plots of CFSE fluorescence of CD4+ (upper panel) or CD8+ (lower panel) after 3 days culture without (blue and green curves, respectively) or with increasing concentrations of ABX464; 15 μM (red curves), 31 μM (yellow curves) or 61 μM (pink curves). CD4+ and CD8+ T cells purified from PBMCs were labeled with CFSE and cultured with the indicated concentration of ABX464 and then analysed by flow cytometry. The plots show the CFSE profiles of viable CFSE-labeled CD4+ and CD8+ T cells. e HIV-1 strain YU2 was used to infect triplicate of monocyte-derived macrophages from different donors in the absence or presence of increasing concentrations of ABX464. Supernatant was harvested 8 days pi and viral capsid protein p24 antigen was quantitated using standard ELISA protocol. Each point represents 9 donors.