Table 2 Summary of IHC labeling of JSRV-infected cells in lung tissue samples from natural cases of OPA (OPA-N) compared with JSRV infected and uninfected ovine lung or lung slices
From: Jaagsiekte sheep retrovirus infection of lung slice cultures
Antibody target antigen | Uninfected lung or lung slices | Tumor cells (OPA-N) | JSRV-positive lung slice cells |
|---|---|---|---|
Cytokeratin | Epithelial cells | Positive | Positive |
JSRV SU | Negative | Positive | Positive |
Club cell specific protein (CCSP) | Club cells in bronchial and bronchiolar epithelium | Negative | Negative |
DC-LAMP | Type II pneumocytes | Mostly positive Mild to intense | Mostly positive Mild to intense |
Ki-67 | Very few cells in lung except macrophages. In lung slice, cells around edges | A few positive cells | Many positive cells |
Phospho-Akt (Ser473) | Bronchiolar epithelial cells and some macrophages | 50-100% positive Mild to intense | 6/6 lung slices positive intense |
Phospho-ERK1/2 (Thr202/Tyr204) | Endothelial cells, some fibroblasts, some bronchiolar cells | All samples positive. Individual OPA nodules varied from mild to intense | 3/6 lung slices positive mild |
Normal rabbit serum | Negative | Negative | Negative |
Mouse IgG | Negative | Negative | Negative |