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Table 1 Summary of antibodies used for IHC

From: Jaagsiekte sheep retrovirus infection of lung slice cultures

Antibody target antigen

Cell target

Dilution

Antibody type

Source

Positive control tissue

Cytokeratin

Pan-respiratory epithelia

1/5000

Mouse mab*

DAKO M3515

normal sheep lung

JSRV Env (SU)

JSRV-infected

1/800

2 Mouse mabs

[16]

OPA positive sheep lung

Club cell specific protein (CCSP)

Club cell

1/20,000

Rabbit polyclonal

[18,19]

normal sheep lung

Dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP)

Type II pneumocytes

1/1,000

Rat mab

Dendritics Clone 1010E1.01

normal sheep lung

Ki-67

Dividing cells

1/1,000

Mouse mab

DAKO M7240

normal sheep lung

Phospho-ERK1/2 (Thr202/Tyr204)

Activated ERK1/2

1/600

Rabbit mab

Cell Signaling NEB(UK) 4370S

normal sheep placenta [62]

Phospho-Akt (Ser473) (D9E)

Activated Akt

1/400

Rabbit mab

Cell Signaling NEB(UK) 4060S

normal sheep placenta [62]

Normal rabbit serum

Negative control

matched to primary

Rabbit polyclonal

In house

NA

Normal mouse IgG

Negative control

matched to primary

Mouse

Sigma I5381

NA

Normal rabbit IgG

Negative control

matched to primary

Rabbit

Sigma I5006

NA

  1. *mab: monoclonal antibody NA: not applicable.