From: Jaagsiekte sheep retrovirus infection of lung slice cultures
Antibody target antigen | Cell target | Dilution | Antibody type | Source | Positive control tissue |
---|---|---|---|---|---|
Cytokeratin | Pan-respiratory epithelia | 1/5000 | Mouse mab* | DAKO M3515 | normal sheep lung |
JSRV Env (SU) | JSRV-infected | 1/800 | 2 Mouse mabs | [16] | OPA positive sheep lung |
Club cell specific protein (CCSP) | Club cell | 1/20,000 | Rabbit polyclonal | normal sheep lung | |
Dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP) | Type II pneumocytes | 1/1,000 | Rat mab | Dendritics Clone 1010E1.01 | normal sheep lung |
Ki-67 | Dividing cells | 1/1,000 | Mouse mab | DAKO M7240 | normal sheep lung |
Phospho-ERK1/2 (Thr202/Tyr204) | Activated ERK1/2 | 1/600 | Rabbit mab | Cell Signaling NEB(UK) 4370S | normal sheep placenta [62] |
Phospho-Akt (Ser473) (D9E) | Activated Akt | 1/400 | Rabbit mab | Cell Signaling NEB(UK) 4060S | normal sheep placenta [62] |
Normal rabbit serum | Negative control | matched to primary | Rabbit polyclonal | In house | NA |
Normal mouse IgG | Negative control | matched to primary | Mouse | Sigma I5381 | NA |
Normal rabbit IgG | Negative control | matched to primary | Rabbit | Sigma I5006 | NA |