Figure 8

Replication of JSRV is more efficient in lung slices from younger sheep. JSRV was added to lung slices obtained from donor animals of different ages and the medium changed daily. The results show JSRV RNA content of 24 h supernatants estimated by RT-qPCR and presented as a percentage of the inoculum. The results represent data from pools of 4 lung slices in each of 3 replicate experiments for each age group. JSRV-∆RT-infected controls are not shown as these were all negative after d 4. Control lung slices without virus were negative for JSRV RNA throughout. The pre-term, new-born and 9 months groups show increasing values with greater dpi, whilst the adult group remains low throughout.