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Figure 4 | Retrovirology

Figure 4

From: Modulation of human endogenous retrovirus (HERV) transcription during persistent and de novo HIV-1 infection

Figure 4

Down-regulation of HIV-1 induced HERV activity by siRNAs targeting HIV-1 transcripts. LC5-HIV cells were treated with the RNAiFect transfection reagent (mock), non-silencing siRNAs (sin.s.) or with siRNAs against gag (sigag), rev (sirev), nef (sinef) and env (sienv). Relative transcript levels of (A) HERV taxa S71pCRTK-1 (group HERV-T), (B) E4-1 (group HERV-E), (C) ERV9 and Seq59 (both group ERV9) and (D) HERV-KC4 (group HERV-K (HML-10)) were determined by qRT-PCR. The Y-axis shows the x-fold relative HERV transcript levels in LC5-HIV cells transfected with non-silencing siRNA (sin.s) and HIV-1-specific siRNAs (sigag, sitat/rev, sinef, sienv) referred to uninfected control cells. The data were normalized to the house-keeping gene RNA Polymerase II (RPII). Standard errors for triplicate experiments are indicated.

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