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Figure 3 | Retrovirology

Figure 3

From: TRIM5α is a SUMO substrate

Figure 3

TRIM5α is SUMO-modified on lysine 10. A. Schematic representation of human and rhesus macaque TRIM5α proteins, indicating the predicted SUMOylated sites, S1 and S2. B. Wild-type hTRIM5α, rhTRIM5α, or the mutants S1m, S2m and S1S2m were in vitro translated, 35S-labelled and used as a substrate for in vitro SUMO-conjugation assay as described in Figure 2. Reaction products were analyzed by SDS-PAGE. C. HeLa cells were transfected with empty vector (ev), wt or S1m rhTRIM5α and transduced two days later with increasing concentrations of HIV-1/GFP. After 48 hours, the proportion of GFP-positive cells was measured by flow cytometry. Graphs shows means of triplicate values +/- SD, representative of three independent experiments.

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