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Table 3 Primers used for RNA in vitro binding assays and locked nucleic acid (LNA) sequences

From: Balanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication

RNA substrate Primer Sequence
Forward #4759 5′ TAA TAC GAC TCA CTA TAG GG AC ATG AGG ATC ACC CAT GTG AAT TCG AAT AGG CGT TAC TCG ACA 3′
Reverse #4761 5′ GGA TGC TTC CAG GGC 3′
LNA Specifity Sequence
αESEtat ESEtat 5′ TTCTTGCTCTCCTCTG 3′
αESE2/ESS2 ESE2/ESS2 5′ TCTAGTCTAGGATCTA 3′
Scrambled - 5′ GACGCGTCCTTACGCG 3′