Table 1 Primers used for cloning
From: Balanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication
Cloned construct | Primer | Sequence |
|---|---|---|
Proviral HIV-1 plasmids | ||
Forward | ||
pNL4-3 ESEtat (mut) | #4709 | 5′ GGG TGT CGA CAT AGC AGA ATA GGC GTT ACT CGA CAT AGG ATA GCA AAA AAT GGA GCC AGT AGA TCC TAG A3′ |
pNL4-3 ESE2 (mut) | #4753 | 5′ GGG TGT CGA CAT AGC AGA ATA GGC GTT ACT CGA CAG AGG AGA GCA AGA AAT GGA GCC AAT CGA TCC TAG ACT AGA GCC CTG G 3′ |
pNL4-3 ESEtat/ESE2 (mut) | #4754 | 5′ GGT GTC GAC ATA GCA GAA TAG GCG TTA CTC GAC ATA GGA TAG CAA AAA ATG GAG CCA ATC GAT CCT AGA CTA GAG CCC TGG 3′ |
Reverse | #640 | 5′ CAA TAC TAC TTC TTG TGG GTT GG 3′ |
SRSF6 expression plasmids | ||
Forward | ||
pCG-SRSF6-HA pCG-RRM1/H-HA pCG-RRM1/L-HA | #4836 | 5′ ATC GTA GAG CAC GCC CG 3′ |
pCG-RRM1-HA | #4971 | 5′ TCC ATA GAA GAC ACC GGG ACC 3′ |
Reverse | ||
pCG-SRSF6-HA | #5085 | 5′ TCT CAG GAT CCT TAC GCG TAA TCA GGA ACA TCG TAT GGG TAA CCA CCA CCA CCA TCT CTG GAA CTC GAC CTG G 3′ |
pCG-RRM1/H-HA | #5084 | 5′ TCA GGA TCC TTA CGC GTA ATC AGG AAC ATC GTA TGG GTA ACC ACC ACC ACC TGG CTT ATC TTC AAT AAG CCT AAT ATT TC 3′ |
pCG-RRM1/L-HA | #5083 | 5′ TCT CAG GAT CCT TAC GCG TAA TCA GGA ACA TCG TAT GGG TAA CCA CCA CCA CCT TCT GTA CGA ACA GGT GGT C 3′ |
pCG-RRM1-HA | #5086 | 5′ TCT CAG GAT CCT TAC GCG TAA TCA GGA ACA TCG TAT GGG TAA CCA CCA CCA CCG CCC CGG GCG TGC TCT A 3′ |