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Table 1 Primers used for cloning

From: Balanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication

Cloned construct Primer Sequence
Proviral HIV-1 plasmids
Forward
pNL4-3 ESEtat (mut) #4709 5′ GGG TGT CGA CAT AGC AGA ATA GGC GTT ACT CGA CAT AGG ATA GCA AAA AAT GGA GCC AGT AGA TCC TAG A3′
pNL4-3 ESE2 (mut) #4753 5′ GGG TGT CGA CAT AGC AGA ATA GGC GTT ACT CGA CAG AGG AGA GCA AGA AAT GGA GCC AAT CGA TCC TAG ACT AGA GCC CTG G 3′
pNL4-3 ESEtat/ESE2 (mut) #4754 5′ GGT GTC GAC ATA GCA GAA TAG GCG TTA CTC GAC ATA GGA TAG CAA AAA ATG GAG CCA ATC GAT CCT AGA CTA GAG CCC TGG 3′
Reverse #640 5′ CAA TAC TAC TTC TTG TGG GTT GG 3′
SRSF6 expression plasmids
Forward
pCG-SRSF6-HA pCG-RRM1/H-HA pCG-RRM1/L-HA #4836 5′ ATC GTA GAG CAC GCC CG 3′
pCG-RRM1-HA #4971 5′ TCC ATA GAA GAC ACC GGG ACC 3′
Reverse
pCG-SRSF6-HA #5085 5′ TCT CAG GAT CCT TAC GCG TAA TCA GGA ACA TCG TAT GGG TAA CCA CCA CCA CCA TCT CTG GAA CTC GAC CTG G 3′
pCG-RRM1/H-HA #5084 5′ TCA GGA TCC TTA CGC GTA ATC AGG AAC ATC GTA TGG GTA ACC ACC ACC ACC TGG CTT ATC TTC AAT AAG CCT AAT ATT TC 3′
pCG-RRM1/L-HA #5083 5′ TCT CAG GAT CCT TAC GCG TAA TCA GGA ACA TCG TAT GGG TAA CCA CCA CCA CCT TCT GTA CGA ACA GGT GGT C 3′
pCG-RRM1-HA #5086 5′ TCT CAG GAT CCT TAC GCG TAA TCA GGA ACA TCG TAT GGG TAA CCA CCA CCA CCG CCC CGG GCG TGC TCT A 3′