Figure 6
From: Integrase inhibitor reversal dynamics indicate unintegrated HIV-1 dna initiate de novo integration
Pre-Integration complex (PIC) is able to cleave and integrate 2-LTRc. MT4 cells were infected with NLENG1-ES-IRES-D116N or NLENG1-ES-IRES-WT +/− 500 nM RAL. 72 hours post-infection, PIC were extracted as described previously [31]. Viral DNA genomes (2-LTRc: white column, DNAi: grey column and DNAL: black column) were quantified before dialysis (D116N; WT; WT + RAL) and after 3 hours (WT ΔRAL) and 6 hours of dialysis (WT ΔRAL; WT + RAL). Results are the mean from three representative independent experiments ± standard deviation (error bars).