Figure 5
From: Integrase inhibitor reversal dynamics indicate unintegrated HIV-1 dna initiate de novo integration
Integrase and other viral and cellular partners are present at the LTR-LTR junction. MT4 cells were infected with NLENG1-ES-IRES-WT in absence (black bars) or in presence of 500 nM RAL (grey bars), or infected with NLENG1-ES-IRES-D116N (white bars). ChIP experiments were conducted without antibodies (NO.AB) or with antibodies against histone H3 (H3), Ku-80 (Ku), LEDGF/p75 (LEDGF), HIV-1-integrase (IN), HIV-1-Reverse-Transcriptase (RT), HIV-1-p24-capside (CA) or HIV-1-matrix-structural-protein (MA) (top) at 24 and 72 hours post-infection. Real-time PCR was used to analyze the nature of the DNA bound to proteins at the LTR-LTR junction (+/−200 bp) (bottom). Arrows highlight the positions of the primers used for quantitative PCR. Results are the mean from three representative independent experiments ± standard deviation (error bars).