2-LTRc are related to the IN catalytic activity. Cells expressing (TC3) or depleted (TL3.4) of LEDGF/p75 were infected with NLENG1-ES-IRES-WT +/− 500 nM RAL or NLENG1-ES-IRES-D116N. (A) Percentage of integration efficiency (upper panel) and 2-LTRc (lower panel) at d3 post-infection for both cell lines. Results are representative of four independent experiments; p value is reported in the histogram. (B) Model explaining the modulation of 2-LTRc amount in different contexts: LEDGF+ (TC3) or LEDGF- (TL3.4) with IN+ (WT) or IN- (D116N or WT + RAL). The numbers above the bars in panel A are related to the corresponding model in panel B.