Figure 2

Reversibility of RAL action. Quantification of total viral DNA at various time post-infection (A-B). (A) MT4 cells were infected on d0 with pNL4-3 WT or D116N (Additional file 1: Figure S2C) +/−RAL (500 nM), +/−SAQ (1 μM). On d3, RAL and/or SAQ were removed (∆RAL or ∆SAQ) or maintained (+); Efavirenz (500 nM) was added (+) or not (−) to the experiment. Black triangle, curve 8: D116N infection; black diamond, curve 9: D116N + SAQ infection. (B) RAL blockade from d0-3 (black diamonds) compared to blockade from d1-3 (open circles). On d3 RAL was removed from all cultures, and the RAL d1-3 culture was diluted 1/10, 1/100 or 1/1000 with uninfected cells or left undiluted. (C) Quantification of DNAi on d3 and d7 for the experiments shown in panels A-B. (D) Quantification of DNAi with RAL added and removed on d0 and d3, respectively. SAQ (1 μM), or T-20 (1 μM) was added on d3, when RAL was removed. AZT (25 μM) was added at d3 when RAL was removed or at d1 and maintained until d7. Results are the mean from three representative independent experiments ± standard deviation (error bars).