Characterization of viral DNA forms during RAL treatment. MT4 cells were infected with HIV-1 eGFP reporter virus (NLENG1-ES-IRES) or its D116N equivalent (p24gag: 40 ng/106 cells). See Additional file 1: Figure S2A-B, and  for details about viruses. At various times after infection, real-time PCR was used to quantify: (A) DNAi and (B) 2-LTRc. (C) Percentages of 2-LTRc over total viral DNA. (D) Inverse correlation between integration inhibition and 2-LTRc formation (72 hours post-infection) in MT4 cells infected with Δenv wild-type virus in the presence of increasing RAL concentrations. Results are the mean from five representative independent experiments ± standard deviation (error bars).