Figure 4
From: The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA
Effect of gRNA/Cas9 treatment on histone modification at HIV-1 LTR. JLat10.6 cells were treated with gRNAs that target HIV-1 pol gene (T5 to T8). Cells were then harvested and subject to ChIP analysis using control IgG, anti-H3, anti-H3K9me3, or anti-H3K36me3 antibodies. Levels of HIV-1 LTR DNA (shown in (A)) or GFP DNA (shown in (B)) in the immunoprecipitated materials were determined by quantitative PCR. Results shown are the average of three independent experiments. Asterisks indicate P < 0.05 (Mann–Whitney U test, SPSS 16.0).