Figure 2

Analysis of mutations in HIV-1 DNA caused by CRISPR/Cas9 treatment. (A) Results of the SURVEYOR assays to measure the NHEJ events resulting from HIV-1 guide RNA treatment. Data of two or three independent transfection experiments are shown. The percentage of the mutated DNA (indicated by arrows) is presented at the bottom of each lane. (B) Mutations at each gRNA target site. The mutated sequences (deletions, insertions and substitutions) are shown in red letters. The PAMs are highlighted in green letters. The wild type HIV-1 DNA is underlined and shown at the top of each aligned sequence panel.