LAG-3 expression on iNKT cell populations. (A) Representative ex vivo surface staining of iNKT cells (CD3 + 6B11+) and sub-gating into CD4+, CD8+ and double negative (DN) subsets. (B) Relative proportion of CD4+ iNKTs within the bulk iNKT population. (C) Representative staining of LAG-3 on CD3 + 6B11+ iNKT cells. (D) LAG-3 expression on bulk, as well as (E) CD8+, DN and CD4+ iNKT populations among study groups. (F) Correlation between LAG-3 expression and CD4 count among HIV+ participants. (G) Correlation and (F) co-expression between CD69 and LAG-3 on iNKT cells among HIV+ participants. (H) Correlation between iNKT LAG-3 expression and plasma IL-10 concentration among all HIV+ and HIV+ ART participants. Statistical comparisons made by Kruskal-Wallis (with Dunn’s post-test), Wilcoxon test and Spearman correlation *p < 0.05, **p < 0.01, ***p < 0.001.